HEMATOLOGICAL PARAMETERS OF FREE-RANGING MOOSE ALCES ALCES (LINNAEUS 1758) (RUMINANTIA, CERVIDAE)
Abstract and keywords
Abstract (English):
Comparative studies that feature the physiology of wild and domestic animals replenish the fundamental knowledge in the field of biology and adaptive potential, thus increasing the efficiency of domestication. Semi-free conditions and artificial environment create prerequisites for epidemics and stress. However, early detection can prevent critical situations. This research provides new data on moose biology and physiology by establishing age and sex hematological parameters. The study featured moose blood samples (n = 55) obtained in the Kirov Region in the northeast of European Russia. Hematological tests relied on a veterinary version of a MicroCC-20 Plus automatic analyzer (High Technology). This research was the first of its kind to introduce a comparative hematological analysis of local European moose according to age and sex. Adults and calves demonstrated significant differences (p < 0.05) in red blood cells, hemoglobin, hematocrit, mean corpuscular volume, mean concentration hemoglobin, mean corpuscular hemoglobin concentration, platelet distribution width, red blood cell distribution width, platelet crit, platelets, leukocytes, and eosinophils. Females and males also had significant differences (p < 0.05) in red blood cells, hemoglobin, mean corpuscular volume, red blood cell distribution width, platelet distribution width, platelets, and eosinophil content. The single- and multivariate analysis made it possible to establish the effect of physiological factors on the blood parameters in moose. The hematological values were in line with the most indicators reported in other publications on wild artiodactyls. The existing differences in blood parameters depended on the species, habitat, food supply, age, and sex.

Keywords:
Alces alces, moose females, moose males, adult animals, calves, hematology, erythrocytes, leukocytes, platelets
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References

1. Litvinov VF, Podoshvelev DA, Kovalev NA. Breeding and resettlement of game animals in Belarus. Relevant Issues of Nature Management, Hunting, and Fur Farming: Proceedings of the International scientific and practical conference dedicated to the 95th anniversary of B.M. Zhitkov All-Russian Research Institute of Hunting and Fur Breeding; 2017; Kirov. Kirov: B.M. Zhitkov All-Russian Research Institute of Hunting and Fur Breeding; 2017. p. 387-390. (In Russ.).

2. Likhatskiy Yu, Likhatskiy E, Atamanov P. Semi-free breeding of ungulates in the Deer nature reserve. Relevant Issues of Nature Management, Hunting, and Fur Farming: Proceedings of the International scientific and practical conference dedicated to the 95th anniversary of B.M. Zhitkov All-Russian Research Institute of Hunting and Fur Breeding; 2017; Kirov. Kirov: B.M. Zhitkov All-Russian Research Institute of Hunting and Fur Breeding; 2017. p. 391-394. (In Russ.).

3. Urosevic IM, Deutz A, Petrovic J, Ristic AZ, Mirceta J. Deer farming in European Union and Serbia: Veterinary legislation perspective. International Symposium on Animal Science 2016; 2016; Belgrade. Belgrade - Zemun: University of Belgrade; 2016. p. 391-398.

4. Veber AEh, Simakov AF, Chuvʹyurova NI, Chalyshev AV, Badlo LP, Kochan TI, et al. Physiology of nutrition and metabolism in moose. Syktyvkar : Komi Scientific Center of the Ural Branch of the Russian Academy of Sciences; 1992. 126 p. (In Russ.).

5. Azaubaeva GS. Blood pattern in animals and birds. Kurgan: Zauralʹe; 2004. 167 p. (In Russ.).

6. Rostal MK, Evans AL, Solberg EJ, Arnemo JM. Hematology and serum chemistry reference ranges of free-ranging moose (Alces alces) in Norway. Journal of Wildlife Diseases. 2012;48(3):548-559. https://doi.org/10.7589/0090-3558-48.3.548

7. Ponder E. The mammalian red cell and the properties of haemolytic systems. Protoplasma. 1935;22:492-494.

8. Knorre EP, Knorre EK. Physiological characteristics of the moose. Proceedings of the Pechoro-Ilych State Reserve. 1959;7:133-167. (In Russ.).

9. Irzhak LI. Moose blood physiology. Proceedings of the Pechoro-Ilych State Reserve. 1964;11:61-66. (In Russ.).

10. Becker SA, Kauffman MJ, Anderson SH. Nutritonal condition of adult female Shiras moose in northwest Wyoming. Alces. 2010;46:151-166.

11. Moyseenko NA. Com ponents of red blood in young moose. Alces. 2002;2:93-97.

12. Ivanter EhV, Korosov AV. Elementary biometrics. Petrozavodsk: PetrGU; 2005. 104 p. (In Russ.).

13. Mogalev NP. Nuclear-cytoplasmic ratio and shape of erythroid cells in reindeer embryos. Archives of Anatomy, Histology, and Embryology. 1987;92(5):45-48. (In Russ.).

14. Chermnykh NA. Ecological physiology of the reindeer. Ekaterinburg: UrO RAN; 2008. 196 p. (In Russ.).

15. Korzhuev PA. Hemoglobin. Comparative Physiology and Biochemistry. Moscow: Nauka; 1964. 287 s. (In Russ.).

16. Korzhuev PA. The respiratory function of blood and the vertebrate skeleton. Uspekhi Sovremennoi Biologii. 1955;39(2):163-195. (In Russ.).

17. Gorodetsky VK. Ecological and physiological parameters of reindeer blood. Abstract cand. bio. sci. diss. Moscow: Moscow Order of Lenin and the Order of the Red Banner of Labor State University. M.V. Lomonosov; 1962. 18 p. (In Russ.).

18. Petrov AK. Moose vs. cattle: growth and development. Abstract dr. bio. sci. diss. Moscow: Moscow Veterinary Academy; 1958. 28 p. (In Russ.).

19. Petrov A. K. Patterns of heart development in moose. Zoologicheskiy Zhurnal. 1961;40(3):447-453. (In Russ.).

20. Irzhak LI. Morphology of mammalian erythrocytes and the age-related changes in red blood cells. Biophysics, Biochemistry, and Pathology of Erythrocytes: Conference proceedings. Krasnoyarsk; 1960. p. 64-68. (In Russ.).

21. Egorova EN, Pustovalova RA, Gorshkova MA. Clinical and diagnostic significance of red blood cell indices, defined automatic hematological analyzers. Volga Medical Journal. 2014;12(3):34-41. (In Russ.).

22. Kochanova NE. Moose metabolism in summer. Proceedings of the Pechoro-Ilych State Reserve. 1964;11:31-54. (In Russ.).

23. Irzhak LI. Physiology of the moose in the Pechoro-Ilychsky Reserve. News of the Komi Branch of the All-Union Geographical Society. 1963;(8):88-89. (In Russ.).

24. Shideler SE, Stoops MA, Gee NA, Tell LA. Hematologic values for Tule elk (Cervus elaphus nannodes). Journal of Wildlife Diseases. 2002;38(3):589-597. https://doi.org/10.7589/0090-3558-38.3.589

25. Kosilov VI, Irgashev TA, Shabunova BK, Akhmedov D. Clinical and hematological parameters of black-spotted cattle of different genotypes and yaks under the mountainous conditions of Tadzhikistan. Izvestia Orenburg State Agrarian University. 2015;51(1):112-115. (In Russ.).

26. Bagirov VA, Aybazov MM, Mamontova TV. Morphometric and biological indicators of the west C. Caucasica. Proceedings of the Stavropol Research Institute of Animal Husbandry and Forage Production. 2014;3(7):33-40. (In Russ.).

27. Novak G, Bodrova L. Hematologic parameters of blood in reindeers with different types of feeding. Bulletin of the BSSA named after V.R. Filippov. 2014;36(3):22-27. (In Russ.).

28. Miller AL, Evans AL, Os Ø, Arnemo JM. Biochemical and hematologic reference values for free-ranging, chemically immobilized wild Norwegian Reindeer (Rangifer tarandus tarandus) during early winter. Journal of Wildlife Diseases. 2013;49(2):221-228. https://doi.org/10.7589/2012-04-115

29. Hochachka P, Somero J. Biochemical adaptation strategy. Moscow: Mir; 1977. 398 p. (In Russ.).

30. Lyakh SP. Adaptation of microorganisms to low temperatures. Moscow: Nauka; 1976. 160 p. (In Russ.).

31. Sroslova GA, Postnova MV, Zimina YuA. Features of adaptation of living organisms. Science of VolSU. Natural Sciences. 2017;7(4):32-38. (In Russ.). https://doi.org/10.15688/jvolsu11.2017.4.5

32. Taylor JA, Feldman BF, Zinki JG, Jain NC. Leukocyte responses in ruminants. In: Feldman BF, Zinkl JG, Jain NC, editors. Schalm’s veterinary hematology. Wiley; 2000. pp. 391-393.

33. Irzhak LI. Respiratory function of blood in mammals. Moscow; Leningrad: Nauka; 1964. 183 p. (In Russ.).

34. Marma BB. Veterinary and physiological observations of moose in a zoo. Proceedings of the Pechoro-Ilych State Reserve. 1967;12:74-86. (In Russ.).

35. Brenner KV, Gurtler H. Further investigations on metabolic and hematological reactions of pigs restraint by means of a rope round the upper jaw. Journal of Experimental Veterinary Medicine. 1981;35:401-407.

36. Kizhina AG, Uzenbaeva LB, Panchenko DV, Ilyukha VA. Morphofunctional organization of blood cells in Nordic Cervidae. Dynamics of the game animals populations in Northern Europe: Book of abstracts. The 7th International symposium; 2018; Petrozavodsk. Petrozavodsk: KRC RAS; 2018. p. 52-54. (In Russ.).

37. Thorn NE, Feldman BF, Zinki JG, Jain NC. Hematology of the deer. In: Feldman BF, Zinkl JG, Jain NC, editors. Schalm’s veterinary hematology. Wiley; 2000. pp. 1179-1183.

38. Vegad JL, Feldman BF, Zinki JG, Jain NC. Normal blood values of the water buffalo (Bubalus bubalis). In: Feldman BF, Zinkl JG, Jain NC, editors. Schalm’s veterinary hematology. Wiley; 2000. pp. 1085-1088.

39. Koryakina LP, Maksimov VI, Machakhtyrov GN. Morphophysiological parameters and enzyme blood profile in domestic reindeer by seasons in the taiga and mountain taiga zones of Yakutia. Agrarian Bulletin of the Urals. 2008;43(1):48-50. (In Russ.).

40. Reshetnikov IS. Effect of natural conditions and seasons on the reindeer thymus lymphocytes. Adaptations to Natural Conditions: Proceedings of the VI All-Union Conference on Ecological Physiology; 1982; Syktyvkar. Syktyvkar: Komi filiala AN SSSR; 1982. p. 46. (In Russ.).

41. Kocan AA, Glenn BL, Thedford TR, Doyle R, Waldrup K, Kubat G, et al. Effects of chemical immobilization on hematologic and serum chemical values in captive white-tailed deer. Journal of the American Veterinary Medical Association. 1981;179(11):1153-1156.

42. Wolfe G, Kocan AA, Thedford TR, Barron SJ. Hematologic and serum chemical values of adult female Rocky Mountain elk from New Mexico and Oklahoma. Journal of Wildlife Diseases. 1982;18(2):223-227. https://doi.org/10.7589/0090-3558-18.2.223

43. Mautz WW, Seal US, Boardman CB. Blood serum analysis of chemically restrained white-tailed deer. Journal of Wildlife Management. 1980;44:343-351.

44. Wesson JA, Sanlon PF, Kirkpatrick RL, Mosby HS. Influence of chemical immobilization and physical restraint on packed cell volume, total protein, glucose, and blood urea nitrogen in blood in white-tailed deer. Canadian Journal of Zoology. 1979;57(4):756-767. https://doi.org/10.1139/z79-093

45. Kazakova MS, Lugovskaya SA, Dolgov VV. The reference values of indicators of total blood analysis of adult working population. Clinical Laboratory Diagnostics. 2012;(6):43-49. (In Russ.).

46. Bleul U, Sorbiraj A, Bostedt H. Effects of duration and storage temperature on cell counts of bovine blood samples as determined by an automated haematology analyser. Comparative Clinical Pathology. 2002;11:211-216. https://doi.org/10.1007/s005800200021


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